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KMID : 1098420110190030162
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Korean Journal of Medicinal Crop Science
2011 Volume.19 No. 3 p.162 ~ p.169
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Development of SCAR Markers for the Authentication of Acori Rhizoma Based on the Analysis of RAPD and Multiplex-PCR
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Moon Byeong-Cheol
Ji Yun-Ui
Lee Young-Mi
Chun Jin-Mi
Lee A-Young
Choo Byung-Kil
Kim Ho-Kyoung
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Abstract
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The rhizomes and herbal medicines originating from Acorus gramineus, A. calamus, A. tatarinowii, and A. gramineus var. pusilus, show significant similarity, and the correct identification of species is very difficult. Random Amplified Polymorphic DNA (RAPD) and Sequence Characterized Amplified Region (SCAR) were used to develop a reliable method for identification of these four species. Several distinct SCAR markers were developed from species-specific RAPD amplicons for each species. Furthermore, a useful molecular marker was established for multiplex-PCR, in order to the four species could be distinguished concurrently. These markers allow efficient and rapid identification of closely-related Acorus species and will be useful for standardization of herbal medicines.
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KEYWORD
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Acorus Species, Random Amplified Polymorphic DNA (RAPD), Sequence Characterized Amplification Region (SCAR), Molecular Marker, Multiplex-PCR
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